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The Patent Office Journal 08/09/2006
15840
(12) PATENT APPLICATION PUBLICATION
(19) INDIA
(21) Application No.: 2104/DEL/2004 A
(22) Date of filing of Application: 26/10/2004
(43) Publication Date: 08/09/2006
(54) Title of the invention: “A PROCESS FOR THE STORAGE OF A CULTURE OR FUNGI.”
(51) International classification
: C 12 N 1/14
(31) Priority Document No
:
(32) Priority Date
:
(33) Name of priority country
:
(86) International Application No and
Filing Date
:
:
(87) International Publication No and
Filing Date
:
(61) Patent of addition to Application No : NA
Filed on
: NA
(62) Divisional to Application No
: NA
Filed on
: NA
(71) Name of Applicant:
THE ENERGY AND RESOURCES
INSTITUTE
THE SECRETARY, DEPARTMENT
OF BIOTECHNOLOGY
Address of the Applicant:
DARBARI SETH BLOCK, HABITAL
PLACE, LODHI ROAD, NEW DELHI
– 110003, INDIA.
BLOCK 2,(7-8 FLOOR) C.G.O.
COMPLEX, LODI ROAD, NEW
DELHI – 110 003, INDIA.
(72) Name of the Inventor:
ALOK ADHOLEYA
KRISHNA SUNDARI
(57) Abstract: The invention relates to a process for the storage of culture, such as mycorrhizae, comprising in the steps of
(i) selecting the fungus, namely Laccaria Laccata; (ii) introducing the fungus into a medium selected from potato dextrose
agar or MMN and having a Ph of 7.0 to 7.5; (ii) allowing the fungus of age; (iv) subjecting the fungus to the step of pre-
freezing in the presence of cryoprotectants such that the temperature of pre-freezing is maintained not above than – 30
o
C,
(v) subjecting the fungus to the step of freeze drying by a tube in tube method at a temperature not above – 100
o
C. Further,
the invention is related to a process for revitalizing freeze dried mycorrhizae which comprises in reconstituting the dried
culture in a rehydrant for a period to reconstitute the culture, placing the disc containing the reconstituted culture on a MMN
agar plate and subjecting it to the step of incubation.
