Animal Cell culture refers to culturing of cells taken from animals, which helps the human race to avoid the usage of experimental animals. This technique helps in mass production of cell products such as immunoglobins, hormones and enzymes.
Total number of patents granted for this technique by USPTO is 11 patents. Some of the patented techniques are given below.
Patent No: 6,593,140 titled ‘Animal cell culture’ with the abstract: An animal cell culture medium is described which contains 2-hydroxy-2, 4, 6-cycloheptatrien-1-one or a derivative thereof to support the growth of animal cells, particularly in agitated cell culture at low iron concentrations.
Patent No: 6,180,401 titled ‘Polypeptide production in animal cell culture’ with the abstract: A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. This is followed by a production phase, where the cultured animal cells of the growth phase are inoculated at a cell seed density of at least 1.0.times.10.sup.6 cells/mL and the cells are cultured at a starting osmolarity of about 400-600 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. Preferably, the glutamine concentration in the cell culture medium is simultaneously controlled in order to curtail production of lactic acid and ammonia which result from unnecessarily high glutamine concentrations. During the growth phase, production of potentially detrimental metabolic waste products, such as lactic acid, is controlled thereby curtailing the increase of osmolality due to accumulation and neutralization of waste products. Thus, the cell growth can be improved. In the production phase, the cell culture conditions are modified in order to arrest or reduce cell growth and thereby direct nutrient utilization toward production, as opposed to cell growth. Overall, it is intended that the method results in an improvement in specific productivity, reduction in production run times and/or an increase in final product concentration.
Patent No: 5,976,833 titled ‘Method for animal cell culture’ with the abstract: The object of the present invention is to provide a method for improving productivity in the production of useful substances by animal cells. The present invention discloses a method for animal cell culture to produce a desired substance, comprising the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.
Patent No: 6,103,529 titled ‘Animal cell culture media comprising peptides derived from rice’ with the abstract: The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of plant derivation, such as at least one plant peptide and/or at least one plant lipid and/or at least one plant fatty acid. The media may further optionally comprise an enzymatic digest or extract of yeast cells. The present invention also provides methods of cultivating animal cells in vitro using these cell culture media formulations. In addition, the media of the present invention can be used for growth of animal cells for virus production. In particular, an animal cell culture medium comprising at least one peptide derived from rice, wherein said animal cell culture medium is completely devoid of animal-derived proteins.
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