Anima Cell Culture is the technique where the cell are seeded and maintained in different cultural conditions.
Some of the patented techniques are given below. This technique helps the human race for optimizing the culturing conditions.
In Patent No: 6,593,140 titled ‘Animal cell culture’ with the abstract: An animal cell culture medium is described which contains 2-hydroxy-2,4,6-cycloheptatrien-1-one or a derivative thereof to support the growth of animal cells,
particularly in agitated cell culture at low iron concentrations.
Patent No: 6,858,428 titled ‘Human and animal cell culture medium containing antithrombin III for suppressing cell death’ with the abstract: The present invention provides low serum or serum free liquid culture media for human or animal cells, and the present invention relates to culture media containing antithrombin III and optionally heparin for the low-serum or serum-free culture of human or animal cells to suppress cell death. The antithrombin III is purified from human, bovine, or other animal serum or antithrombin III derived from recombinant bacteria or cells containing the full length of the antithrombin III gene.
Patent No: 6,818,625 titled ‘Method for increasing survival rate of cells in animal cell culture under hypoxia condition’ with the abstract: The present invention relates to a method for increasing survival rate of cells in animal cell culture under hypoxia condition by adding antibiotics to the culture media. The method of present invention comprises a step of culturing animal cells in culture media containing antibacterial agent of quinolones, quinones, aminoglycosides or chloramphenicol at the concentration range of 0.1 to 1000 .mu.g/ml. The invented method can be practically applied for high-density animal cell culture to produce recombinant proteins or cultured cells.
Patent No: 6,180,401 titled ‘Polypeptide production in animal cell culture’ with the abstract: A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. This is followed by a production phase, where the cultured animal cells of the growth phase are inoculated at a cell seed density of at least 1.0.times.10.sup.6 cells/mL and the cells are cultured at a starting osmolarity of about 400-600 mOsm in the presence of a concentration of glucose controlled throughout the culturing to be within a range between about 0.01 and 1 g/L. Preferably, the glutamine concentration in the cell culture medium is simultaneously controlled in order to curtail production of lactic acid and ammonia which result from unnecessarily high glutamine concentrations. During the growth phase, production of potentially detrimental metabolic waste products, such as lactic acid, is controlled thereby curtailing the increase of osmolality due to accumulation and neutralization of waste products. Thus, the cell growth can be improved. In the production phase, the cell culture conditions are modified in order to arrest or reduce cell growth and thereby direct nutrient utilization toward production, as opposed to cell growth. Overall, it is intended that the method results in an improvement in specific productivity, reduction in production run times and/or an increase in final product concentration.
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