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Animal Cell Biotechnology

In US Patent No. 7132271 titled Methods for enhancing the production of viral vaccines in cell culture the patent referred to an article by MacDonald et al., "Development of new cell lines for animal cell biotechnology", Critical Reviews Biotech. (1990) 10:155-178.

MacDonald et al., "Development of new cell lines for animal cell biotechnology", Critical Reviews Biotech. (1990) 10:155-178. was cited in US Patent No. 7,125,706 titled Method for the production and purification of adenoviral vectors. The abstract of that patent reads as follows: The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge. The specification in US Patent No. 7,094,400 titled Transkaryotic implantation reads as follows: The desired or effector gene sequence is preferably isolated and cloned onto a plasmid prior to being incubated with the cells. It is, however, also possible to incubate the cells with an unfractionated collection of plasmids each of which contains a different gene sequence or solely with the desired or effector gene sequence. If unfractionated plasmids are used, it is desirable to screen the resulting transfected cells for a cell which contains and expresses the desired gene sequences. Such a cell would then preferably be purified from the other transfected cells by known and commonly used techniques prior to being introduced into the subject recipient. Techniques for cell culture are extensively disclosed by Freshney, R. I. (In: Culture of Animal Cells, A Manual of Basic Technique) (Aln R. Liss, Inc., NY, pp. 55 78 (1983)) and Lambert, K. J., et al. (In: Animal Cell Biotechnology, Vol. 1, Spier, R. E., et al., Eds., Academic Press, NY, pp. 86 122 (1985)), which references are hereby incorporated by reference.

Some of the other US Patents that deal with this subject are given below.

7,087,412 Methods for large scale protein production in prokaryotes 7,077,839 Methods for tissue welding using laser-activated protein solders 7,038,015 Interferon gamma polypeptide variants 6,955,910 Method for large scale production of recombinant DNA-derived TPA or K2S molecules which refers to Cartwright, T., "Production of tPA from Animal Cell Cultures," In Animal Cell Biotechnology, vol. 5, R.E. Spier and J.B. Griffiths (eds.), Academic Press, N.Y. p. 217-245 (1992). .

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