Antisense RNA

Browse Indian Patents from 2005 by Weekly Journals

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Antisense RNA refers to the nucleotide, which are complementary to the particular mRNA from which it is synthesized. This technique is used to investigate the function of unknown genes. It is also used to find how genes in the plants coordinate their activity.

Some of the interesting patented works are listed below. Total number of patents granted o this by USPTO is 6 patents.

Patent No: 6,943,022 titled ‘Inhibition of apoptosis by the expression of antisense RNA of caspase-3’ with the abstract: The present invention relates to an antisense nucleotide of caspase-3, an expression vector encoding antisense RNA of caspase-3, and an inhibition method of cellular apoptosis in recombinant Chinese hamster ovary (CHO) cells using the same. More precisely, the present invention relates to an antisense nucleotide composed of a base sequence represented by Sequence ID No. 1, which suppresses the expression of caspase-3, an expression vector containing the above antisense nucleotide and expressing antisense RNA of caspase-3, and an inhibition method of apoptosis of recombinant cells by suppressing the expression of caspase-3 through introducing the above expression vector into the recombinant cells and then expressing antisense RNA of caspase-3. According to the present invention, apoptosis can be inhibited by suppressing the activation of caspase-3, which is related to the apoptosis, and the integrity of target protein produced in the recombinant cells can be enhanced. Patent No: 6,776,986 titled ‘Inhibition of HIV-1 replication by antisense RNA expression’ with the abstract : Novel antisense sequences to the unspliced or single spliced portions of mRNA transcript from HIV-1 provirus, optionally co-expressed with an inhibitory transdominant mutant HIV-1 protein, are found to be useful in the treatment of HIV-1 infection.

Patent No: 6,686,192 titled ‘Antisense RNA expression strategies effective against Streptococcus thermophilus bacteriophages’ with the abstract: Recombinant bacteria containing nucleotide sequences encoding Streptococcus thermophilus phage helicase and primase genes expressed in the antisense orientation and methods of making and using the same are disclosed. Replication of bacteriophage infecting this bacterial cell, can be inhibited or decreased by constitutively expressing, the antisense oligonucleotide encoding for S. thermophilus phage helicase or primase.

Patent No: 5,908,779 titled ‘Targeted RNA degradation using nuclear antisense RNA’ with the abstract: The invention pertains to a novel method and construct for regulating gene expression through inhibition of gene expression by nuclear antisense RNA. The construct provides an efficient means for introducing, expressing and accumulating the antisense RNA in the nucleus. The antisense RNA hybridizes to the sense mRNA in the nucleus, thereby preventing both processing and cytoplasmic transport. The construct comprises a promoter, antisense sequences, and a cis- or trans-ribozyme which generates 3'-ends independently of the polyadenylation machinery and thereby inhibits the transport of the RNA molecule to the cytoplasm. The construct may also comprise a histone stem-loop structure that assists in stabilizing the transcripts against exonucleolytic degradation.

Patent No: 5,882,880 titled ‘Human checkpoint gene and gene for antisense RNA thereof’ with the abstract: A novel human radiation protecting checkpoint (RAP-1) gene and the RAP-1 protein encoded by the gene are disclosed. RAP-1 is believed to be involved in the regulation of cell cycle progression and/or programmed cell death (apoptosis). A human antisense RNA which can bind to the mRNA of the human RAP-1 protein, as well as the DNA from which the antisense RNA is transcribed are also disclosed. Additionally, a method for isolating DNA damage-monitoring checkpoint genes is described. The use of the DNA and RNA sequences and of the protein of the invention for the early detection, prevention and/or treatment of cancer, AIDS and other diseases is also discussed.