Gene silencing refers to the switching of mechanism of a particular gene other than the gene mutation. The humans use this technique to silence the gene responsible for genetic disorders.
Total number of patents granted for this method is 9 patents. Some of these patented techniques are given below with abstracts.
Patent no: 6,753,139 titled ‘Gene silencing’ with the abstract: Methods are disclosed for screening for the occurrence of gene silencing (e.g., post transcriptional gene silencing) in an organism. Also provided are methods for isolating silencing agents so identified.
Patent no: 7,109,393 titled ‘Methods of gene silencing using inverted repeat sequences’ with the abstract: The present invention provides methods for inhibiting target gene expression, by expressing in a cell a nucleic acid construct comprising an inverted repeat and a sense or antisense region having substantial sequence identity to a target gene, wherein the inverted repeat is unrelated to the target gene.
Patent no: 7,012,172 titled ‘Virus induced gene silencing in plants’ with the abstract: The present invention discloses methods for interfering with expression of the genes in plant cells by using replicating recombinant viral vectors. A host plant is infected at one or more locations with a recombinant viral vector. The vector is both an initiator and a target of the RNA-triggered gene silencing in plant cells. The vector upon infection is capable of directing self-replication and producing a transcription product of a nucleic acid segment. The transcription product interferes with the expression of a specific gene in plant cells.
Patent no : 7,001,739 titled ‘Isolation of proteins involved in posttranscriptional gene silencing and methods of use’ with the abstract : The present invention includes a method for detecting and isolating sugarcane proteins that interact with the HC-Pro and P1 proteins of SrMV and other proteins involved in gene silencing, particularly in sugarcane. The method uses a two hybrid assay with an HC-Pro, P1, or other silencing-related protein-containing bait protein and a prey protein containing a polypeptide encoded by a DNA molecule in a cDNA library. The method also includes identification of false positives through reverse two-hybrid assays and using in vitro techniques such as farwestern blots or pull down assays where plant physiological conditions may be replicated. Finally, interactions may be confirmed in planta. Some novel proteins used in and discovered using the these methods are also identified. Methods of using viral and plant proteins to regulate silencing in plants such as sugarcane are also discussed.
Patent no: 6,972,349 titled ‘Control of post-transcriptional gene silencing in plants’ with the abstract: Calmodulin-like polypeptides named rgs-CaM are disclosed. cDNAs coding rgs-CaM are also provided. In addition, methods of using rgs-CaM cDNAs and polypeptides for modulating gene expression in plants are also provided.
Patent no: 6,635,805 titled ‘Methods and DNA constructs for gene silencing in transgenic plants’ with the abstract: The invention presents DNA constructs comprising a promoter operably linked to DNA which can be transcribed in a plant cell to an RNA transcript, wherein the RNA transcript comprises plant virus sequence from an RNA virus which confers on the RNA transcript the ability to replicate in the cytoplasm of the plant cell, wherein the transcript lacks all or part of the viral genome not required for replication in the cytoplasm, and further comprises at least one targeting sequence which is foreign to the plant virus sequence and causes post-transcriptional gene silencing of one or more target genes. The invention also presents methods to use the DNA construct to cause post-transcriptional gene silencing of a target gene in a plant.
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