Purification of proteins is the process where the proteins are purified using different techniques. Some of the USPTO patented techniques are mentioned below.
Patent no: 5,905,143 titled ‘Purification of proteins’ with the abstract: When proteins are purified using a protein-binding dye immobilized on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. An ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein to overcome the protein of the leaking dye.
Patent no: 6,518,406 titled ‘Method for purification of proteins’ with the abstract: The present invention relates to methods for purification of antithrombin-III (AT-III) by precipitation of impurities. The said methods comprise (a) adding, to a solution comprising antithrombin-III, a saccharide and citrate, in an amount sufficient for impurities to precipitate while antithrombin-III essentially remains in solution; (b) allowing impurities to precipitate; and (c) removing the precipitated impurities, thereby obtaining a solution comprising purified antithrombin-III. The invention also relates to pharmaceutical compositions, obtainable by the said methods, comprising purified antithrombin-III, as well as to reconstituted pharmaceutical compositions essentially free from visible particles.
Patent no: 6,342,362 titled ‘Methods and compositions for the purification of proteins or other macromolecules’ with the abstract: The present method relates to improvements in methods for the purification of proteins and other macromolecules. In particular, the invention relates to the use of a tag to permit the facile repurification of a previously purified macromolecule, after the tagged macromolecule has been allowed to interact with other compositions. These methods can be useful in the modification of macromolecules and in screens involving macromolecules, as well as for the purification of portions of an isolated macromolecule.
Patent no: 4,857,317 titled ‘Process for the extraction and purification of proteins from culture media producing them’ with the abstract: The process is applicable to the supernatant of engineered yeast cells disrupted in the presence of a non-ionic detergent; it comprises the precipitation of contaminants by polyethylene glycol and the treatment of this latter supernatant with either a bivalent metal cation or, after eventual ultrafiltration, with ammonium sulfate.
Patent no: 4,667,018 titled ‘Process for the purification of proteins using acidic polysaccharide gels’ with the abstract: A process for the purification of proteins from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising passing the liquid medium over acidic polysaccharide gel particles of at least 0.5 mm in the shortest dimension, and recovering the desired proteins from the gel particles by elution with an aqueous salt solution having ions concentration of at least 5 grams per liter.
Patent no: 5,179,196 titled ‘Purification of proteins employing CTAP-III fusions’ with the abstract: The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.
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