Three dimensional tissue modeling method involves in the production of artificial tissues and organs. Some of the patented methods are given below.
Patent no: 6,998,017 titled ‘Methods of making a three-dimensional tissue’ with the abstract An improved method for imprinting a paper web during a wet pressing event is disclosed which results in asymmetrical protrusions corresponding to the deflection conduits of a deflection member. In one embodiment, differential velocity transfer during a pressing event serves to improve the molding and imprinting of a web with a deflection member. Improved deflection members are also disclosed. Improved tissue webs produced are also disclosed having useful sets of physical and geometrical properties, such as a pattern densified network and a repeating pattern of protrusions having asymmetrical structures.
Patent no: 6,218,182 titled ‘Method for culturing three-dimensional tissue in diffusion gradient bioreactor and use thereof’ with the abstract : A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media; allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.
Patent no: 6,943,021 titled ‘Three dimensional vaginal tissue model containing immune cells’ with the abstract : Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.
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